1. Restriction Endonuclease enzymes play a very important role in modern molecular cloning techniques.2. A restriction endonuclease functions by " scanning " the length of a DNA molecule. 3. An example of a Type I restriction endonuclease . 4. However, if genomic DNA is treated with a particular restriction endonuclease many DSBs will be created. 5. Most commonly used restriction enzymes are Type II restriction endonuclease ( See article on Restriction enzymes for examples ). 6. The plasmid contains a genetic sequence corresponding to the recognition site of a restriction endonuclease , such as EcoR1. 7. Sickle-cell gene mutation probably arose spontaneously in different geographic areas, as suggested by restriction endonuclease analysis. 8. When examining the preferential double strand cleavage of DNA, the restriction endonuclease PstI bind to pSM1 plasmid DNA. 9. The sequence altered by the mutation eliminates the recognition site for the restriction endonuclease MstII that recognizes the nucleotide sequence. 10. Ronald W . Davis first discovered sticky ends as the product of the action of EcoRI, the restriction endonuclease .
मोबाइल